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The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:
• Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
• Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
• Simple—easy to use, with a single reagent kit for all cell types
• Versatile—open system allows electroporation parameters to be optimized freely
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The Agilent 6460 Triple Quadrupole LC/MS delivers superior sensitivity for trace level analysis with performance specifications in
Signal-to-Noise ratio (S/N) and Instrument Detection Limit (IDL). IDL is a rigorous, statistically based metric that indicates practical
sensitivity performance of your quantitative assays. The Agilent 6460 Triple Quadrupole LC/MS achieves sensitivity and resolution
specifications with autotune.
Gentian violet slightly stains the nuclei of the leucocytes. The blood specimen is diluted 1:20 in a WBC pipette with the diluting fluid and the cells are counted under low power of the microscope by using a counting chamber. The number of cells in undiluted blood is reported per cumm (µl) of whole blood.
A Spectrophotometer is an analytical instrument used to identify materials including organic polymers. Infrared spectrophotometers record the relative amount of energy as a function of the wavelength/frequency of the infrared radiation when it passes through a sample. Therefore, chemical structures of different samples will reflect differences in the IR absorption spectrum allowing for identification of a sample. Unlike a dispersive spectrometer, an FTIR spectrometer or FTIR spectrophotometer is used to simultaneously obtain spectral data of a sample. It does this by using an interferometer to collect the interferogram, also known as the raw data/signal format, which can then be translated into the infrared spectrum of the sample by means of a fourier transform algorithm. As a result there are many advantages including greater signal-to-noise ratio, high resolution, higher throughput, and a short wavelength limit. FTIR spectrometers can be used in a variety of industries including environmental, pharmaceutical, and petrochemical.
Laminar Flow Cabinet – is used only for sample protection. Laminar Flow Cabinet is a work bench or similar enclosure, which creates a particle-free working environment by taking air through a filtration system and exhausting it across a work surface in a laminar or unidirectional air stream.
Autoclaves are also known as steam sterilizers and are typically used for healthcare or industrial applications. An autoclave is a machine that uses steam under pressure to kill harmful bacteria, viruses, fungi, and spores on items that are placed inside a pressure vessel. The items are heated to an appropriate sterilization temperature for a given amount of time. The moisture in the steam efficiently transfers heat to the items to destroy the protein structure of the bacteria and spores.
The costs of an autoclave can vary greatly because of the various uses and applications of this technology. Industrial and pharmaceutical autoclaves are customized and manufactured for a specific use and therefore costs are likely to differ compared to autoclaves you find in a hospital or dental office.
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